Strain Typing of Microorganisms

Map changes to your facility's organisms with DiversiLab rep-PCR exclusively from AAIPharma Services

New rep-PCR technology offers genetically based strain typing of organisms for microbial identification. The DiversiLab system, available from AAIPharma Services through a partnership with bioMérieux, is more accurate than currently available alternatives, because it amplifies non-coding repetitive sequences interspersed throughout the bacterial genome using Polymerase Chain Reaction (PCR).

Strain typing of bacterial and fungal organisms offers a more exact genetic identification, and can be used as a tool to map and build a library of a facility's microbial organisms. The data generated can be used for trending and tracking seasonal changes of a facility's indigenous microorganisms over time. Results are available on a secure website with 24-hour access.

Benefits of strain typing

Environmental monitoring programs can benefit by building a library of organisms. Each isolate can be stored in the library and later compared to one another or compared to isolates in the AAIPharma library. This provides accurate and trending data for companies to know if an organism is changing or adapting to the environment.

Strain typing is also ideal for manufacturers that require the exact same strain of a microorganism be used to make a product. Biotech companies and brewers are examples of industries needing to ensure they are using the same microorganism each time.

The AAIPharma Services laboratory facility in St. Louis is mapped as a resource for identifying out-of-spec situations and seasonal changes. The secure website is CFR Part 11 compliant and operated by an independent host, guaranteeing client confidentiality.

USP has proposed a new chapter in PF 35(1), <1113> Microbial Identification, which gives guidance for microbial identification and discusses genetic identifications. This is quickly becoming an industry expectation for microbial identifications.

How it works

The strain typing of a microorganism starts with an accurate Gram stain. The isolate must then be identified accordingly using the Vitek 2 Compact to speciate bacteria and yeast. Mold is identified to the genus level by microscopic identification. Isolates correctly identified can then have strain typing performed.

The number of isolates cannot be mixed among bacteria, yeast and mold due to different preparation procedures for each. They are priced as only bacteria, yeast or mold isolates.

For more information, including pricing, call +1 732 346 5100 (New Jersey) or +1 314 487 6776 (St. Louis) or contact AAIPharma Services.